5902022
BIOCATALYSIS AND ENZYMOLOGY
,
BIOCATALYSIS AND ENZYMOLOGY
, 2 ov
Lecturer info
Senior Lecturer UWE MUNSTER.
Lectures and exercises:
Lectures 22 h, laboratory exercises 30 h
Lecture room and time
Monday 12 - 14, FC117
Tuesday 12 - 14, FC117
Weekly teaching / period |
|
|
|
|
|
Lectures (h): |
- |
- |
- |
4 |
- |
Exercises (h): |
- |
- |
- |
x |
- |
Course objectives
Training and learning principal techniques and tools in bio-catalysis and enzymology, protein structure, its functions and its role in environmental biotechnology.
Content of the course
This course introduces the student to the role of biocatalysis and enzymes in environmentally relevant processes such as in nutrient mobilization and its cycling, in the degradation of bio- and geopolymers, in detoxification and transformation of pollutants in soil, sediment and water. Measurements, techniques and approaches in enzymology, principles in enzyme kinetics and transformation tools in characterizing enzyme reaction mechanisms. Mechanisms of enzyme induction, repression and derepression. Tracing of biocatalysis in environmentally relevant bioprocesses with chromogenic- and fluorogenic model substrates under in situ conditions. A lab-exercise course will be provided as an intensive course at the end of the lecture period.
Requirements
Written examination and approved exercises.
Literature
A. Fersht: Structure and Mechanism in Protein Science;W. Freeman & Co., New York, 1999, C. Branden & J. Tooze: Introduction to Protein Structure; Garling Publ., New York, 1991, N. C. Price & L. Stevens: Fundamentals of Enzymology; Oxford University Press, 1999, R. Eisenthal & M. J. Danson: Enzyme Assays; IRL Press, Oxford, 1992.
Information on prerequisites
Courses in Chemistry, Biochemistry, Microbiology, Environmental Sciences, Instrumental Bioanalysis
Prerequisites
Number |
Name |
|
|
5902020 |
2 |
Obl. |
Notes
Laboratory exercises will be organised as one week intensive period. The time and venue will be informed later.