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BIO-2726 GENETIC ENGINEERING AND FERMENTATION TECHNOLOGIES, 4 cr
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Courses persons responsible
Matti Karp
Lecturers
Matti Karp
Ville Santala
Katariina Tolvanen
Nina Virolainen
Implementations
| Period 1 | Period 2 | Period 3 | Period 4 | Period 5 | Summer | |
| Exercise work | - | - | - | - | 40 h/week | - |
| Exam | ||||||
Objectives
Modern biotechnology research utilizes genetic engineering to enhance process functioning for higher yields and improved down-stream processing. To master these needs the student has to learn the rationale how to solve some of the key points from upstream to down-stream.
Content
| Content | Core content | Complementary knowledge | Specialist knowledge |
| 1. | The course is a hands-on laboratory exercise on basic methodologies in recombinant-DNA technologies and related fermentation technologies. The works consist of cloning of genes by polymerase chain reaction (PCR) into Eschericia coli expression vectors containing protein purification tags. The correct constructs will be verified by various molecular biology tools. Thereafter the over-producing E. coli strains are inoculated into a lab-scale fermentor and the growing kinetics and factors affecting the growth are studied. Finally the cells are collected and protein expressed will be purified by affinity chromatography. Education in the methodology related to protein analytical chemistries are provided throughout the course, too. |
Evaluation criteria for the course
Prerequisites
| Code | Course | Credits | M/R |
| BIO-2000 | BIO-2000 Molecular Biotechnology | 4 | Mandatory |
| BIO-2601 | BIO-2601 Methods in Genetic Engineering | 4 | Mandatory |
| BIO-5900 | BIO-5900 Reactor Design and Modelling | 3 | Mandatory |
Prequisite relations (Sign up to TUT Intranet required)
Additional information about prerequisites
For international students prerequisite is an exam from the book: Glick, B.R. and Pasternak, J.J., Molecular Biotechnology.
Remarks
The course is suggested for 3-4 year biotechnology or MSc Programme in Science and Bioengineering students. The course is organized as intensive course, with duration of 2 weeks. The timetable will be announced later.
| Last modified | 22.08.2007 |
| Modified by | Saila Kallioinen |