Course Catalog 2010-2011
Basic

Basic Pori International Postgraduate Open University

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Course Catalog 2010-2011

SGN-6126 Cell culturing, microscopy and cell image analysis, 3 cr

Person responsible

Andre Sanches Ribeiro, Olli Yli-Harja

Requirements

Final project work (40% of the final grade). Final reports on the experimental work and the results obtained from the image analysis (60%). To pass the course, the student is required to: a) Execute the final project and deliver three reports on the experimental works. b) Attend at least 80% of the lessons.

Learning outcomes

From this course the student will know how to prepare standard bacterial cultures and will have basic knowledge of how to operate microscopes. The student will also be introduced to state of the art software for cell image analysis and profiling. Finally, the students will gain insight on the interpretation of the results and will obtain knowledge on the efficiency of the algorithms when applied to real data. After the course, the student will be able to: 1) Identify and define experimental techniques related to bacterial culturing and cell imaging. Demonstrate the ability to apply these methods to extract information from biological systems. 2) Interpret data generated from the microscope, classify strengths and weaknesses of the bright field and fluorescence measurements, summarize results of the measurements and explain the connection between measurements and underlying biological processes. 3) Implement experimental techniques and apply them to extract data from biological systems. Calculate statistical properties of the features measured at the single cell level. Apply knowledge of existing software to extract the relevant information. 4) Analyze results of the measurements. Compare the various methodologies used for measuring a variable such as cell fluorescence levels. 5) Compare and appraise different algorithms for extracting quantifiable features from the images, and interpret the results from the measurements. 6) Create and develop new features that would improve the algorithms for specific goals.

Content

Content Core content Complementary knowledge Specialist knowledge
1. Principles of cell culturing of bacteria and microscopy     
2. Experimental cell culturing of bacteria     
3. Imaging fluorescent bacteria under the microscope     
4. State of the art tools for cell images analysis     
5. Segmentation and extraction of information about the cells using CellProfiler     

Evaluation criteria for the course

Project work (40% of the final grade). Final reports on the experimental work and the results obtained from the image analysis (60%). To pass the course, the student is required to: a) Execute the project work and deliver three reports on the experimental works. b) Attend at least 80% of the lessons.

Assessment scale:

Numerical evaluation scale (1-5) will be used on the course

Study material

Type Name Author ISBN URL Edition, availability, ... Examination material Language
Book           Molecular Biology of the Cell, 4th edition, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter.      English  
Journal           Fermino, A. M., Fay, F. S., Fogarty, K. and Singer, R. H., Visualization of single RNA transcripts in situ, Science, 280, 1998, 585-9.      English  
Journal           Reue, K., mRNA quantitation techniques: considerations for experimental design and application, J. Nutr. 128, 1998, 2038-44.      English  
Lecture slides   Lecture slides              English  
Other online content   CellProfiler         Carpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, Guertin DA, Chang JH, Lindquist RA, Moffat J, Golland P, Sabatini DM (2006) CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biology 7:R100.      English  
Other online content   ImageJ     http://rsb.info.nih.gov/ij/     Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/, 1997-2009.      English  

Additional information about prerequisites
Basic knowledge of biology/systems biology. Introduction to Computational Systems Biology (SGN-6056) or equivalent. Also, SGN-1157 Signal Processing Basics or SGN-1201 Signaalinkäsittelyn menetelmät.

Prerequisite relations (Requires logging in to POP)

Correspondence of content

There is no equivalence with any other courses

Additional information

This course is lectured every year. Course webpage: http://www.cs.tut.fi/~sanchesr/SGN-6216/index.htm
Suitable for postgraduate studies

Last modified21.02.2011