Course unit, curriculum year 2024–2025
KEM.361
Chromatography, 3 cr
Tampere University
- Description
- Completion options
Teaching periods
Active in period 3 (1.1.2025–2.3.2025)
Course code
KEM.361Language of instruction
EnglishAcademic years
2024–2025, 2025–2026, 2026–2027Level of study
Advanced studiesGrading scale
General scale, 0-5Persons responsible
Responsible teacher:
Alexander EfimovResponsible organisation
Faculty of Engineering and Natural Sciences 100 %
Coordinating organisation
Environmental Engineering and Chemistry Studies 100 %
Core content
- Application area of modern chromathography. Separation principles. Analytical and preparative methods. Chromatography types and modes (TLC, LC, HPLC, GC). Stationary and mobile phases used for different analytes. Basic retention dependencies in GC, LC, SEC.
- Theory of chromatography. Van-Deemter equation.
- Chromatography instrumentation. Detectors for GC and HPLC. Qualitative and quantitative analysis. Retention parameters.
- Practice in qualitative analysis by TLC, preparative separation by LC, quantitative analysis by GC and HPLC.
- Advanced knowledge of retention dependencies.
- Separation efficiency dependencies.
- Choice of the optimal detection method for particular analyte based on selectivity and sensitivity.
- Optimization of separation conditions.
Learning outcomes
Prerequisites
Compulsory prerequisites
Recommended prerequisites
Further information
Learning material
Equivalences
Studies that include this course
Completion option 1
Completion of all options is required.
Exam
No scheduled teaching
Participation in teaching
07.01.2025 – 23.02.2025
Active in period 3 (1.1.2025–2.3.2025)