Birhanu Assefa Belay’s dissertation presents novel three-dimensional (3D) imaging techniques to enhance the study of complex cell cultures that resemble physiological conditions. As biomedical research shifts from simpler 2D cell cultures to complex 3D systems, such as organ-on-chips and microphysiological systems, there is a growing demand for advanced imaging methods that enable accurate characterization.
Traditional imaging often falls short in analyzing these 3D models, so Birhanu developed new methods that allow researchers to observe cellular structures and function deep within large cell cultures. These techniques offer a non-invasive, label- free high-resolution 3D imaging at mesoscopic depths (1 to 10 mm) and provide both quantitative and qualitative insights into how cells interact with their environment. This is crucial for fields like cellular biophysics and tissue engineering.
“This research provides essential tools for understanding cell behavior in realistic, three-dimensional settings,” Birhanu explains.
His work not only enhances our ability to study 3D cell cultures but also supports potential applications in biomedical research, including improved methods for studying disease models and drug screening.
Public defence on Monday 2 December
The doctoral dissertation of MSc Birhanu Assefa Belay in the field of biomedical engineering titled Advances in Optical Imaging Techniques for in vitro 3D Cell Culture and Mechanotransduction Studies will be publicly examined at the Faculty of Medicine and Health Technology at Tampere University at 13:00 on Monday, 2 December 2024, in the Jarmo Visakorpi auditorium of the Arvo building, (Arvo Ylpön katu 34, Tampere). The Opponent will be Professor Pekka Hänninen from the University of Turku. The Custos will be Professor Jari Hyttinen from Tampere University.