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Course Catalog 2010-2011
BIO-2726 Genetic engineering and fermentation technologies, 4 cr |
Person responsible
Matti Karp, Suvi Santala, Ville Santala, Nina Virolainen
Lessons
| Study type | P1 | P2 | P3 | P4 | Summer | Implementations | Lecture times and places |
| |
Requirements
The course includes weekly meetings in which students will present their results. No exam is included but a written report of the work is mandatory.
Completion parts must belong to the same implementation
Learning outcomes
After passing the laboratory course the student can understand in practice how modern biotechnology research utilizes genetic engineering to enhance process for higher yields and improved down-stream processing. She/he will learn the rationale how to solve some of the key points from upstream to down-stream by genetic engineering. After the course, the student can carry out gene cloning, expression in host organism, protein purification via affinity chromatography and understand methods to analyze the protein of interest. By writing a scientific report the student will learn to summarize, rationalize and explain the scientific results.
Content
| Content | Core content | Complementary knowledge | Specialist knowledge |
| 1. | Methods in gene cloning | ||
| 2. | Recombinant protein expression in Escherichia coli | ||
| 3. | Protein purification | ||
| 4. | Methods of protein analysis | ||
| 5. | Compiling a scientific report, drawing conclusions from results and discussing |
Evaluation criteria for the course
The grade (0-5) will be determined by the extent of self-initiative, diligence and activeness of the student during the laboratory course. The quality of the scientific report also affects the grade. Meeting the schedule set for writing the report is of high importance. Attendance is expected at laboratory experiments.
Assessment scale:
Evaluation scale passed/failed will be used on the course
Partial passing:
Prerequisites
| Course | Mandatory/Advisable | Description |
| BIO-2601 Methods in Genetic Engineering | Mandatory | 1 |
| BIO-2406 Microbial genetics | Mandatory |
1 . For international students, the mandatory prerequisite is BIO-2456 Laboratory course on macromolecules: structure
Additional information about prerequisites
For international students mandatory prerequisites include an exam from the book: Glick, B.R. and Pasternak, J.J., Molecular biotechnology.
Prerequisite relations (Requires logging in to POP)
Correspondence of content
There is no equivalence with any other courses
Additional information
The course is suggested for 3-4 year Biotechnology or MSc Programme in Science and Bioengineering students.
The course is organized as intensive course, with duration of 2 weeks.
More precise information per implementation
| Implementation | Description | Methods of instruction | Implementation |
| Laboratory assignments |
Contact teaching: 66 % Distance learning: 0 % Self-directed learning: 34 % |