KEB-23726 Laboratory Course on Industrial Biotechnology, 4 cr
Additional information
The course is organized as intensive laboratory course, with duration of 2 weeks.
The course is only intended for degree students
Suitable for postgraduate studies
Person responsible
Ville Santala
Lessons
Implementation 1: KEB-23726 2015-01
| Study type | P1 | P2 | P3 | P4 | Summer |
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Requirements
Mandatory laboratory work and written report. No exam.
Completion parts must belong to the same implementation
Learning Outcomes
After passing the laboratory course the student can understand in practice how modern biotechnology research utilizes genetic engineering to enhance process for higher yields and improved down-stream processing. She/he will learn the rationale how to solve some of the key points from upstream to down-stream by genetic engineering. After the course, the student can carry out gene cloning, expression in host organism, protein purification via affinity chromatography and understand methods to analyze the protein of interest. By writing a scientific report the student will learn to summarize, rationalize and explain the scientific results.
Content
| Content | Core content | Complementary knowledge | Specialist knowledge |
| 1. | Methods in gene cloning | High-throughput gene cloning | |
| 2. | Recombinant protein expression in Escherichia coli | Bioprocess optimization | Fed-batch cultures |
| 3. | Protein purification | Methods of protein analysis | |
| 4. | Compiling a scientific report, drawing conclusions from results and discussing | Critical analysis of obtained results |
Instructions for students on how to achieve the learning outcomes
The grade (0-5) will be determined by the extent of self-initiative, diligence and activeness of the student during the laboratory course. The quality of the scientific report also affects the grade. Meeting the schedule set for writing the report is of high importance. Attendance is expected at laboratory experiments.
Assessment scale:
Numerical evaluation scale (1-5) will be used on the course
Partial passing:
Prerequisites
| Course | Mandatory/Advisable | Description |
| KEB-23736 Industrial and Molecular Biotechnology | Mandatory | 1 |
1 . or corresponding course(s)
Correspondence of content
| Course | Corresponds course | Description |
| KEB-23726 Laboratory Course on Industrial Biotechnology, 4 cr | BIO-2726 Genetic engineering and fermentation technologies, 4 cr |