BMT-53808 Methods in Single Cell Biology, 5 cr

Implementation BMT-53808 2019-01

Description

The student will be introduced to a variety of experimental techniques and analysis tools in quantitative, single cell biology studies. The aim is to provide understanding on techniques of single cell observation and on how to interpret the results. The focus of this course will be the manipulation and measurements of gene expression in prokaryotic systems using live cell multi-modal microscopy systems and flow cytometry. The model organism will be Escherichia coli. Finally, students will gain understanding of the objectives, applicability and limitations of the methods used in quantitative single cell biology studies. Several of the lectures will be in a laboratory, so that the students become knowledgeable in handling the devices, such as microscopes and flow cytometers.

Lessons

Assessment scale

Evaluation scale passed/failed will be used on the course

Requirements

To pass the course, the student is required to:
a) Pass in all grading requisites.
b) Attend at least 80% of the lessons.
c) In each lecture, deliver a 1-2 pages report on the previous lecture.
d) Pass the final assignment (project and its 30 min presentation in the last lecture).

Additional information of course implementation

Only 6 students can attend the course every year, due to space limitations in the laboratory. The following criteria will be used to select which students will attend the course. From those enrolled and attending the first lecture, we will select according to the order of enrollment in the course. If you do not attend the first lecture, the next person in the list will be given priority. The order of enrollment is visible in POP.

https://sites.google.com/view/andreribeirolab/home/teaching