BMT-53808 Methods in Single Cell Biology, 5 cr
Additional information
Additional information about the course is provided at: https://sites.google.com/view/andreribeirolab/home/teaching
Suitable for postgraduate studies.
Person responsible
Andre Sanches Ribeiro
Lessons
| Implementation | Period | Person responsible | Requirements |
| BMT-53808 2019-01 | 2 |
Andre Sanches Ribeiro |
To pass the course, the student is required to: a) Pass in all grading requisites. b) Attend at least 80% of the lessons. c) In each lecture, deliver a 1-2 pages report on the previous lecture. d) Pass the final assignment (project and its 30 min presentation in the last lecture). |
Learning Outcomes
The student will be introduced to a variety of experimental techniques and analysis tools in quantitative, single cell biology studies. The aim is to provide understanding on techniques of single cell observation and on how to interpret the results. The focus of this course will be the manipulation and measurements of gene expression in prokaryotic systems using live cell multi-modal microscopy systems and flow cytometry. The model organism will be Escherichia coli. Finally, students will gain understanding of the objectives, applicability and limitations of the methods used in quantitative single cell biology studies. Several of the lectures will be in a laboratory, so that the students become knowledgeable in handling the devices, such as microscopes and flow cytometers. The classes will be held at: D502, Arvo Building
Content
| Content | Core content | Complementary knowledge | Specialist knowledge |
| 1. | The concept of cell to cell diversity | ||
| 2. | Techniques for single-cell measurements of RNA and proteins | ||
| 3. | Model organisms and constructs (plasmids and vectors), Gene transfer techniques | ||
| 4. | Basics of Microscopy | ||
| 5. | Basics of Flow Cytometry | ||
| 6. | Basics of single-cell image analysis | ||
| 7. | Project presentations |
Additional information about prerequisites
Basic knowledge of molecular biology and/or systems biology.
Correspondence of content
| Course | Corresponds course | Description |
| BMT-53808 Methods in Single Cell Biology, 5 cr | BMT-53807 Methods in Single Cell Biology, 3 cr |